| Congresso Brasileiro de Microbiologia 2023 | Resumo: 856-1 | ||||
Resumo:Biosurfactants are metabolites synthesized by microorganisms with an amphipathic molecular configuration, which have applicability in several industrial sectors. For a stored bioproduct, shelf life is an important factor, since biological products can be sensitive to several environmental factors, which can influence their stability over time. In this sense, the objective of the present work was to evaluate the stability of the surfactant properties of a fungal biosurfactant (BS), patented (BR1020200235931) as an inducer of the rooting of a halophyte plant, stored for a period of four years. For the stability tests, 1% of lyophilized BS after four years of shelf life was used, diluted in distilled water as a value referring to its CMC (critical micellar concentration), which corresponds to the smallest amount of BS necessary to reduce the maximum surface tension of the medium. The analysis of the effect of temperature was performed with the thermal treatment of the BS at the following temperatures: 4, 60 and 121 °C, over 1 hour of exposure. To evaluate the effect of salinity on the stability of the SB, it was subjected to different concentrations of NaCl: 2, 15 and 30% and, finally, the pH of the solution containing the BS was adjusted to 4, 7 and 9, in order to evaluate the effect of pH variations on the surfactant properties of the biomolecule. After subjecting the BS to these extreme conditions of salinity, pH and temperature, the surface tension and emulsification index were measured after 24 h of exposure. The results of measuring surface tension and emulsification indexes did not exceed 5 units under the tested conditions, with no significant changes in the surfactant capacity of the biomolecule. With this, the excellent performance of the BS of M. circinelloides against extreme environmental conditions of temperature, salinity and pH, after a period of 4 years of shelf life, was proven. Therefore, the potential applicability of BS for the agricultural sector is confirmed, as it is effective in soils with different levels of acidity, temperature and salinity, as well as in the bioremediation of marine environments. Thus, studying the influence of environmental factors on the stability of bioproducts is crucial to ensure their quality, safety and efficacy, as well as to promote sustainability and technological innovation in various industrial sectors. Palavras-chave: biosurfactant, agriculture, filamentous fungus Agência de fomento:Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||